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为了研究心脏型脂肪酸结合蛋白(H-FABP)基因在滩羊不同时期不同部位表达量,分析其与肌内脂肪(IMF)含量的关系,试验建立了检测滩羊H-FABP mRNA表达水平的实时荧光定量RT-PCR方法,并采用该方法对2,4,6,9,12月龄滩羊(各20只,雌雄各半)的背最长肌、腰大肌和股二头肌的肌肉组织H-FABP mRNA表达水平进行了检测,还测定了三个部位肌肉组织IMF的含量。结果表明:性别与活重、胴体重、IMF、H-FABP mRNA背部肌肉表达量和股部肌肉表达量差异均不显著(P≥0.05)。不同月龄、不同部位H-FABP基因的表达水平总体上呈上升-下降-上升的趋势,且2月龄表达量最高(P<0.01)。三个部位IMF沉积规律基本相同,在生长前期随着月龄的增长其IMF含量呈上升趋势,6月龄左右上升明显,表达量为腰大肌﹥股二头肌﹥背最长肌。月龄与活重、胴体重和股部表达量差异极显著(P≤0.01),但与IMF、背部和腰部表达量差异不显著(P≥0.05)。2月龄滩羊背最长肌中H-FABP基因表达量与IMF含量相关系数为0.104(P=0.327),呈正相关;股二头肌H-FABP基因表达量与IMF相关系数为0.773(P<0.01),呈强度正相关;腰大肌2~12月龄期间HFABP基因表达量与IMF含量呈正相关,相关系数为0.125。说明H-FABP基因在不同组织中表达量不同,股二头肌中的表达量最高,腰大肌次之,再次为背最长肌,且腰大肌的表达量仅次于股二头肌。
In order to study the expression of H-FABP gene in different parts of Tan sheep during different periods and to analyze the relationship between H-FABP mRNA and intramuscular fat (IMF) content, a real-time The quantitative RT-PCR method was used to detect the muscle of the back longus muscle, the psoas muscle and the biceps femoris of 2, 4, 6, 9, 12 months old Tan sheep (each 20, male and female) Tissue H-FABP mRNA expression levels were detected, but also determined the three parts of the muscle tissue IMF content. The results showed that there was no significant difference in sex and live weight, carcass weight, IMF and H-FABP mRNA expression between the back and the femoral muscles (P> 0.05). The expression level of H-FABP gene at different months and months showed a general trend of increasing-decreasing-increasing, with the highest expression at 2 months (P <0.01). The IMF deposition patterns in the three areas were basically the same. The IMF content increased with the increase of age at the early growth stage, and increased significantly at about 6 months of age. The expression level was psoas> biceps> dorsal longissimus muscle. There was significant difference between month old and live weight, carcass weight and femur (P≤0.01), but no significant difference with IMF, back and waist (P≥0.05). The correlation coefficient between H-FABP gene expression and IMF content in 2-month-old goat longest muscle was 0.104 (P = 0.327), which was positively correlated. The correlation coefficient between H-FABP gene expression and IMF was 0.773 (P <0.01). There was a positive correlation between HFABP gene expression and IMF content in 2 ~ 12 months of psoas muscle, with a correlation coefficient of 0.125. H-FABP gene expression in different tissues in different amounts, the highest expression in the biceps femoris, followed by the psoas muscle, again the longest muscle, and psoas expression after the biceps femoris .