目的采用凝胶法检测CIK细胞上清液及混悬液中的细菌内毒素。方法采用《中国药典》2010年版二部附录下XIE细菌内毒素的检测方法。结果 CIK细胞稀释4倍后的上清液及稀释8倍后的混悬液对细菌内毒素的检测结果均无干扰,其内毒素的限值符合规定。结论采用细菌内毒素检查法可定性检测CIK细胞上清液及其混悬液中的内毒素进行。 Objective To detect the bacterial endotoxin in CIK cell supernatant and suspension by gel method. Methods The detection method of bacterial endotoxins of XIE under the appendix of 2010 edition of Chinese Pharmacopoeia was adopted. Results The supernatant after 4-fold dilution of CIK cells and the suspension after 8-fold dilution did not interfere with the detection results of bacterial endotoxin, and the limit of endotoxin in the CIK cells was within the limits. Conclusion Bacterial endotoxin test can qualitatively detect endotoxin in CIK cell supernatant and its suspension.