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为进一步研究腺苷三磷酸抑制癌细胞增殖诱导分化的作用机理，用ＡＴＰ作用于人横纹肌肉瘤单细胞克隆亚系ＲＤＬ６细胞，用免疫荧光细胞化学方法观察ＡＴＰ作用后的ＲＤＬ６细胞核内Ｐ２１蛋白表达增强，ｃ－ｍｙｃ蛋白表达降低，膜下胞质内微丝束－应力纤维组装改善，粘着斑内的主要粘附蛋白——纽蛋白（ｖｉｎｃｕｌｉｎ）表达增强，正常小鼠成肌细胞Ｃ２Ｃ１２细胞核内Ｐ２１蛋白高表达，ｃ－ｍｙｃ蛋白不表达。结果表明：ＲＤＬ６细胞Ｐ２１表达的丢失和ｃ－ｍｙｃ表达的活化与细胞的增殖和分化异常相关，从而表明Ｐ２１表达增强和ｃ－ｍｙｃ表达的降低在ＲＤＬ６细胞表型逆转过程中起重要作用。
To further study the mechanism of adenosine triphosphate inhibition of cancer cell proliferation-induced differentiation, ATP was used to act on RDL6 cells of human rhabdomyosarcoma clonal sublines, and the expression of P21 protein in RDL6 nuclei after ATP treatment was observed by immunofluorescence cytochemistry The expression of c-myc protein was decreased, and the assembly of microfilament-stress fibers in the submicron cytoplasm was improved. The expression of vinculin, the main adhesion protein in the adherent, was enhanced. Normal mouse myoblast C2C12 cell nuclear P21 The protein is highly expressed and the c-myc protein is not expressed. The results showed that the loss of P21 expression and the activation of c-myc in RDL6 cells were associated with abnormal cell proliferation and differentiation, indicating that enhanced expression of P21 and decreased c-myc expression play an important role in the reversal of RDL6 cell phenotypes.