目的　研究视网膜色素上皮 (retinal pigmentepithelium,RPE)细胞中增生细胞核抗原 (pro-liferating cell nuclear antigen,PCNA)表达及其反义寡核苷酸 (antisense oligodeoxynucleotides,AS- ODN)对其表达和细胞增生的抑制作用 ,为增生性玻璃体视网膜病变 (proliferative vitreoretinopathy,PVR)治疗探索基因治疗新途径。　方法　 (1)体外培养兔眼 RPE细胞 ,在不同时间采用链霉亲合素 -生物素化过氧化物酶复合物 (streptoavidin- biotin- enzyme complex,SABC)免疫组织化学法检测 PCNA的表达 ;(2 )脂质体介导下不同浓度的 PCNA AS- ODN和正义寡核苷酸 (sense oligodeoxynucleotides,S- ODN)分别作用于体外培养的 RPE细胞 ,采用免疫组织化学方法检测 PCNA的表达 ;(3)四唑盐比色法 (methyl thiazolyl tetra-zolium,MTT)检测在不同浓度的 PCNA AS- ODN和 S- ODN作用下 RPE细胞生长活性及其生长抑制率。　结果　 (1)体外培养兔眼 RPE细胞表达 PCNA,表达高峰为培养后 4 8h;(2 )在 0 .2 8、1.12 μmol/ LAS- ODN作用下 ,PCNA的表达明显受抑制 ;(3) 0 .2 8、1.12μmol/ L的 PCNA AS- ODN能明显抑制 RPE细胞增生活性 ,并呈剂量依赖性 ,其生长抑制率分别达 5 3%、81%。　结论　一定浓度 PCNA AS- ODN能序列特异? Objective To investigate the expression of PCNA in retinal pigmentepithelium (RPE) cells and the effect of antisense oligodeoxynucleotides (AS-ODN) on its expression and cell proliferation Inhibition and explore new ways of gene therapy for the treatment of proliferative vitreoretinopathy (PVR). Methods (1) Rabbit eye RPE cells were cultured in vitro and the expression of PCNA was detected by streptavidin-biotin-enzyme complex (SABC) immunohistochemistry at different time. 2) PCNA AS-ODN and sense oligodeoxynucleotides (S-ODN) were transfected into RPE cells cultured in vitro and detected by immunohistochemistry. ) Methylthiazolyl tetra-zolium (MTT) was used to detect the growth activity and growth inhibition rate of RPE cells under different concentrations of PCNA AS-ODN and S-ODN. Results (1) The expression of PCNA in cultured RPE cells in vitro was peaked at 48 h after culture. (2) The expression of PCNA was significantly inhibited by 0.28,1.12 μmol / L AS-ODN. (3) .2,8,1.12μmol / L PCNA AS-ODN can significantly inhibit the proliferation of RPE cells in a dose-dependent manner, with the growth inhibition rates of 53% and 81%, respectively. Conclusion A certain concentration of PCNA AS-ODN sequence specific?