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赤霉素3-氧化酶(GA3ox)是赤霉素生物合成途径中的一种关键酶,在茎的伸长中起着重要的作用。为探讨GA3ox基因和Rht12矮秆小麦矮化之间的关系,本研究利用同源克隆方法分离得到了小麦品种中国春和宁98-2105(含矮秆基因Rht12)的GA3ox1和GA3ox2基因,并对其在茎的不同部位进行了表达模式分析。研究结果表明,小麦GA3ox1和GA3ox2均含有3个外显子和2个内含子,其编码的蛋白具有2-酮戊二酸依赖性的双加氧酶典型的保守结构域,但中国春和宁98-2105的GA3ox1和GA3ox2之间存在部分碱基的差异。实时定量表达分析结果显示,GA3ox1和GA3ox2在中国春和宁98-2105茎的不同部位的表达趋势基本一致,但表达量具有较大差异,GA3ox1和GA3ox2在宁98-2105茎的倒一节结节中的转录水平显著高于中国春,推测宁98-2105中赤霉素的信号转导通路可能受到了影响,即矮秆基因Rht12可能影响了赤霉素信号的传导。本研究结果为进一步研究矮秆基因Rht12的致矮机理和利用提供了理论依据。
Gibberellin 3-oxidase (GA3ox) is a key enzyme in the gibberellin biosynthesis pathway and plays an important role in stem elongation. In order to investigate the relationship between GA3ox gene and dwarfing of Rht12 in wheat, GA3ox1 and GA3ox2 genes were cloned by homologous cloning from Chinese spring and Ning 98-2105 (including dwarfing gene Rht12) It is expressed in different parts of the stem pattern analysis. The results showed that wheat three GA3ox1 and GA3ox2 contain three exons and two introns, the encoded protein has a 2-ketoglutarate-dependent dioxygenase typical conserved domain, but the Chinese spring and There is a partial base difference between GA3ox1 and GA3ox2 in Ning 98-2105. The results of real-time quantitative analysis showed that the expression patterns of GA3ox1 and GA3ox2 in different parts of Chinese Spring and Ning 98-2105 stems were basically the same, but the expression levels were quite different. GA3ox1 and GA3ox2 expressed in the inverted nodules of Ning 98-2105 The transcriptional level in the node was significantly higher than that in the Chinese spring. It is speculated that the signal transduction pathway of gibberellin in Ning 98-2105 might be affected. The dwarfing gene Rht12 may affect the signal transduction of gibberellin. The results of this study provide a theoretical basis for further research on the dwarfing mechanism and utilization of dwarfing gene Rht12.