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黑木耳菌种的培育,分原菌繁育和母菌培育两个阶段。原菌繁育包括原菌分离、一级菌种培养基配制、灭杂菌、接种、二级菌种扩制五道工序;母菌培育包括培养基配制、装瓶、灭杂菌、接种、培养五道工序。原菌繁育1.原菌分离。有两种方法:①担孢子弹射分离法:选择朵大、肉厚、皱摺多、健壮的新鲜木耳或当年采收的干木耳,用清水浸泡,发胀后洗净,放在消过毒的三角瓶内,再用无菌水振荡冲洗3次,取出放进灭过菌
Cultivation of black fungus species, sub-bacteria breeding and mother mold two stages. Incubation of the original bacteria, including the separation of bacteria, a strain of medium preparation, off bacteria, inoculation, secondary expansion of five species of bacteria; mother mold culture including medium preparation, bottling, sterilization, inoculation, culture Five processes. Original breeding 1. Isolation of bacteria. There are two methods: (1) basidiospora ejection method: choose large, fleshy, wrinkled and more, robust fresh fungus or dry fungus harvested that year, soaked with water, swollen and washed on the disinfection The triangular flask, and then washed with sterilized water oscillation 3 times, remove and put in sterilization bacteria