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用成年雄性Ｗｉｓｔａｒ大鼠切断一侧视神经后，随即将自体坐骨神经段、视神经段或挤压后的坐骨神经段植入眼球玻璃体内，植入的神经段长２ｍｍ。术后动物分别存活２、４或８周。将动物麻醉处死后，取同侧视网膜制备切片，Ｎｉｓｓｌ染色，观察机网膜节细胞（ＲＧＣ）的变化，并用计算机图像分析仪测量ＲＧＣ胞体截面积。结果表明，植入坐骨神经段各组ＲＧＣ大多数存活，并出现巨大的节细胞。存活２周时，ＲＧＣ胞体截面积均值比正常组大；４周时胞体进一步增大；８周时则有所下降。在同一存活期内，增加移植神经段数目或植入存活卫周的挤压坐骨神经，ＲＧＣ胞体截面积增大更明显；超过５０μｍ２的巨大节细胞细胞数在本组的构成比也增大。
After an optic nerve was cut off in an adult male Wistar rat, the sciatic nerve segment, the optic nerve segment or the compressed sciatic nerve segment was immediately implanted into the vitreous body. The implanted nerve segment was 2 mm in length. Postoperative animals survived 2, 4 or 8 weeks respectively. After anesthesia was sacrificed, the ipsilateral retina was prepared and Nissl staining was performed to observe the change of RGC. The cross-sectional area of RGC cell body was measured by computer image analyzer. The results showed that most of the RGCs in the sciatic nerve graft group survived and showed giant ganglion cells. Survival of 2 weeks, RGC cell body cross-sectional area of the mean value than the normal group; 4 weeks the body cell body further increased; then decreased at 8 weeks. In the same survival period, increasing the number of grafted nerve segments or implanted scapulohumeral perivascular sciatic nerve, RGC cell body cross-sectional area increased more obvious; more than 50μm2 giant cell cell number in this group also increased the composition ratio.