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以酪蛋白为底物测定了中华鳖胰脏、胃、肠等组织蛋白酶活性、最适ｐＨ和最适底物浓度。结果表明：胃、胰组织蛋白酶活性显著高于肠组织蛋白酶活性，且胃＞胰＞前肠＞中肠＞后肠；胃、胰和肠组织蛋白酶最适ｐＨ分别为２．２、６．２、７．６；胃、前肠组织蛋白酶最适底物浓度分别为１．２％、０．８％。
Using casein as substrate, the cathepsin activity, optimal pH and optimal substrate concentration of pancreas, stomach and intestine of Chinese soft-shelled turtle were determined. The results showed that the activity of protease in stomach and pancreas was significantly higher than that in intestinal tissue, and the stomach> pancreas> foregut> midgut> hindgut; the optimum pH of protease in stomach, pancreas and intestine were 2.2, 6.2 , 7.6; the optimum concentration of protease in stomach and foregut were 1.2% and 0.8% respectively.