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目的对云南部分地区鸽粪分离株新型隐球菌进行多位点微卫星位点(multilocus microsatellite typing,MLMT)分型,了解其种群分布特征。方法对分离自云南省部分地区鸽粪中的152株新型隐球菌提取DNA,分别以CNG1、CNG2、CNG3为引物进行PCR扩增并测序,计算每一菌株CNG1中TA、CNG2中GA以及CNG3中CAT的重复数,结合不同基序重复数进行MLMT分型。结果采用MLMT法将大理、昭通、曲靖和临沧地区鸽粪分离株新型隐球菌格鲁比变种分为6个基因型,其中MLMT-17型占91.45%,为主要基因型;MLMT-26、29、34、39、40型分别占0.66%、1.97%、0.66%、0.66%和4.60%。结论 MLMT-17是云南省部分地区鸽粪新型隐球菌格鲁比变种中最常见的基因型。
Objective To investigate the distribution of Cryptococcus neoformans by multilocus microsatellite typing (MLMT) in some areas of Yunnan. Methods DNA was extracted from 152 strains of Cryptococcus neoformans isolated from pigeon feces in Yunnan province. The CNG1, CNG2 and CNG3 primers were used for PCR amplification and sequencing. CAT repeat number, combined with different motifs repeat MLMT typing. Results The genotypes of C. difficile of Cryptococcus neoformans in Dali, Zhaotong, Qujing and Lincang were classified into 6 genotypes by MLMT method, MLMT-17 accounted for 91.45% of the total genotype. MLMT-26, 29, 34, 39 and 40 accounted for 0.66%, 1.97%, 0.66%, 0.66% and 4.60% respectively. Conclusion MLMT-17 is the most common genotype of Geluobi variant of Cryptococcus neoformans in some areas of Yunnan Province.