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监测大麦黄矮病毒株系类型、分布及其变化是研究小麦黄矮病流行测报、抗病性鉴定、培育抗耐品种的重要前提。1988年利用生物学与血清学技术对我国冬、春及夏播小麦区小麦黄矮病田间病株标样进行了监测研究。本文着重报道两种检测的结果。 一.材料与方法 小麦黄矮病病株标样的采集:在发病季节(4-8月)到河南、陕西、甘肃、内蒙、河北等冬、春麦及夏播小麦地区的地块隨机采样。每株采摘2-3片叶,病叶放在塑料袋内并存入冰瓶保存。 传毒介体准备:将麦二叉蚜Schizaphis graminum Rond(Sg)、麦长管蚜Sitobion(Macrosiphum)avenae Fab(Ma)和禾谷缢管蚜Rhopalosiphum padi L.(Rp)在隔离的养虫室内的小麦苗上繁殖。 抗血清来源:GPV株系抗血清为本组制备;MAV与Mix(PAV+RPV)抗血清分别由美国Dr.Rochow和澳大利亚Dr.Waterhouse提供。 监测步骤:经1.生物学试验(见中国农业
Monitoring barley yellow dwarf virus strain types, distribution and changes of wheat yellow dwarf epidemic test reported, the identification of disease resistance, an important prerequisite for breeding resistant varieties. In 1988 using biological and serological techniques in winter, spring and summer sowing wheat wheat yellow dwarf disease in field samples were monitored. This article focuses on the results of the two tests. I. Materials and methods Collection of wheat yellow dwarf disease samples: In the season (April-August) to Henan, Shaanxi, Gansu, Inner Mongolia, Hebei and other winter, spring wheat and summer sowing wheat plots random sampling . Each plant picked 2-3 leaves, diseased leaves in plastic bags and stored in ice bottles. Preparation of Toxoplasma vectors: Schizaphis graminum Rond (Sg), Sitobion (Macrosiphum) avenae Fab (Ma), and Rhopalosiphum padi L. (Rp) were isolated in isolated shelters Of wheat seedlings breeding. Antisera origin: GPV strain antisera were prepared in this group; MAV and Mix (PAV + RPV) antiserum were supplied by Dr. Rochow, USA and Dr. Waterhouse, Australia respectively. Monitoring steps: 1. Biology experiments (see China Agriculture