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目的研究谷胱甘肽S转移酶A1(Glutathinoe S-transferase A1,GSTA1)基因多态性与无锡地区汉族妇女中子癇前期发病的相关性。方法随机抽取就诊于无锡市妇幼保健院产科的子癇前期产妇90例作为实验组,正常妊娠产妇90例作为对照组,采用限制性片段长度多态性聚合酶链反应(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)检测两组产妇血液中位于GSTA1基因启动子区的SNP位点rs3957357(GSTA1-69C/T)的等位基因型及基因型。结果 GSTA1-69C/T中T等位基因在实验组和对照组中的频率分别为12.2%及13.3%,两组差异无统计学意义(χ~2=0.0997,P=0.752);同样,该多态性位点的三种基因型CC、CT、TT在实验组中的分布频率为77.8%、20.0%、2.2%,在对照组中的分布频率分别为74.5%、24.4%、1.1%,差异无统计学意义(χ~2=0.7990,P=0.691)。结论GSTA1基因多态性与无锡地区汉族妇女子癇前期的发病无相关性
Objective To investigate the association of Glutathione S-transferase A1 (GSTA1) gene polymorphism with the incidence of preeclampsia in Han women in Wuxi area. Methods Totally 90 pregnant women with preeclampsia who were treated in Wuxi Maternity and Child Health Hospital were randomly selected as the experimental group and 90 normal pregnant women as the control group. Polymerase chain reaction-restriction fragment length polymorphism, PCR-RFLP) were used to detect allele and genotype of SNP locus rs3957357 (GSTA1-69C / T) located in the promoter region of GSTA1 gene in maternal blood. Results The frequencies of T allele in GSTA1-69C / T were 12.2% and 13.3% in the experimental and control groups, respectively, with no significant difference between the two groups (χ ~ 2 = 0.0997, P = 0.752) The distribution frequencies of three genotypes CC, CT, TT in the polymorphic loci were 77.8%, 20.0% and 2.2% in the experimental group and 74.5%, 24.4% and 1.1% respectively in the control group, The difference was not statistically significant (χ ~ 2 = 0.7990, P = 0.691). Conclusion There is no correlation between GSTA1 gene polymorphism and the incidence of preeclampsia in Han women in Wuxi