论文部分内容阅读
目的:探讨125I治疗人脑胶质瘤的可行性及其机制。方法:体外培养人胶质瘤细胞(SHG-44),采用MTT法检测125I粒子对SHG-44细胞增殖抑制的影响;采用立体定向的方法建立大鼠脑内人胶质瘤模型,接种1周后经MRI检测脑内形成实体瘤。将建模成功大鼠随机分成实验组(n=30)与对照组(n=10)。实验组大鼠在当天肿瘤区域植入125I粒子1粒,植入粒子1周后复查MRI,2周后处死大鼠,取对照组、实验组肿瘤周边组织及肿瘤组织做增殖细胞核抗原(PCNA)免疫组化检测。结果:MTT法检测SHG-44细胞经125I粒子照射后,增殖受到明显抑制,接种1周后脑内形成实体瘤;125I可抑制肿瘤细胞生长,延长荷瘤鼠生存期,PCNA基因表达受抑制。结论:125I抑制体外培养的SHG-44细胞生长和颅内接种的脑胶质瘤生长的作用机制可能与抑制PCNA基因表达有关。
Objective: To investigate the feasibility and mechanism of 125I treatment of human glioma. Methods: The human glioma cells (SHG-44) were cultured in vitro. The effect of 125I particles on the proliferation inhibition of SHG-44 cells was detected by MTT assay. The rat glioma model was established by stereotactic method. After the brain MRI detection of solid tumors. The successful model rats were randomly divided into experimental group (n = 30) and control group (n = 10). Rats in the experimental group were implanted with one 125I particle in the tumor area on the same day. MRI was performed one week after the implantation of the particles. Two weeks later, the rats were sacrificed and the proliferating cell nuclear antigen (PCNA) was taken from the control and experimental groups. Immunohistochemistry. Results: The proliferation of SHG-44 cells was inhibited by 125I irradiation after MTT assay. One week after inoculation, SHG-44 cells formed solid tumors in the brain. 125I inhibited the growth of tumor cells, prolonged the survival of tumor-bearing mice and inhibited the expression of PCNA gene. CONCLUSION: The mechanism by which 125I inhibits the growth of SHG-44 cells cultured in vitro and the intracranial inoculation of glioma may be related to the inhibition of PCNA gene expression.