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目的与方法：采用常规玻璃微电极技术研究丹皮酚对离体心肌细胞自律性（ＡＭ）、延迟后除极（ＤＡＤ） 及触发活动（ＴＡ）的影响。结果：１．８×１０－４ｍｏｌ／Ｌ丹皮酚灌流组，肾上腺素（Ａｄｒ）的阈浓度空白对照组为（１．２８±０．５７）μｍｏｌ／Ｌ，药后为（１．５６±０．５３）μｍｏｌ／Ｌ（ｎ＝９，Ｐ＞０．０５）；用（１．８×１０－ ３） ｍｏｌ／Ｌ丹皮酚（Ｐａｅ）灌流组，Ａｄｒ 浓度由空白对照组的（１．２２ ±０．６２）μｍｏｌ／Ｌ升高到（６．２２±２．１１）μｍｏｌ／Ｌ（ｎ＝９，Ｐ＜０．０１）。１．８×１０－３ｍｏｌ／Ｌ的Ｐａｅ 能明显抑制哇巴因（Ｏｕａ）诱发的ＤＡＤ的幅值，当基本刺激周长为５００，４００，３００ 和２００ ｍｓ 时，其ＤＡＤ幅值从（５．５±２．０）ｍＶ，（７．３±２．１）ｍＶ，（８．０ ±２．４）ｍＶ和（９．２±１．９）ｍＶ减小到（３．０±１．１）ｍＶ、（３．６±１．７）ｍＶ，（４．３±２．０） ｍＶ和（５．９ ±１．６） ｍＶ，Ｐ＜０．０１。当基本刺激周长为２００ ｍｓ时，ＴＡ 数目由５．５±１．０ 降至０．７±０．３（Ｐ＜０．０１）。结论：丹皮酚能抑制心肌细胞ＡＭ、ＤＡＤ及ＴＡ，具有抗心律失常作用
Objective and Method: To study the effect of paeonol on isolated myocardial cell autonomy (AM), post-delayed depolarization (DAD) and trigger activity (TA) using conventional glass microelectrode technique. Results: The threshold concentration of Adr in the 1.8×10-4 mol/L paeonol perfusion group was (1.28±0.57) μmol/L in the blank control group and (1.56±) after drug administration. 0.53) μmol/L (n=9, P>0.05); (1.8×10 −3) mol/L paeonol (Pae) perfusion group, Adr concentration from the blank control group (1 .22 ± 0.62) μmol/L increased to (6.22 ± 2.11) μmol/L (n = 9, P <0.01). Pae of 1.8×10-3 mol/L significantly inhibited the amplitude of Oua-induced DAD. When the basic stimulus circumference was 500, 400, 300 and 200 ms, the DAD amplitude was from (5). (5 ± 2.0) mV, (7.3 ± 2.1) mV, (8.0 ± 2.4) mV and (9.2 ± 1.9) mV reduced to (3.0 ± 1) .1) mV, (3.6 ± 1.7) mV, (4.3 ± 2.0) mV and (5.9 ± 1.6) mV, P <0.01. When the basic stimulus circumference was 200 ms, the number of TA decreased from 5.5±1.0 to 0.7±0.3 (P<0.01). Conclusion: Paeonol can inhibit AM, DAD and TA in myocardial cells and has antiarrhythmic effect