癌肿组织的器官培养,可为抗癌药物的筛选、肿瘤细胞生物学特性、肿瘤细胞逆转分化等方面研究提供较为理想的模型。这方面的研究国外已有不少报道(Tchao等,1968;Ellison等,1969;Yar-nell和Ambrose,1969a,b;Rubinstein等,1973;Wolff和Wolff 1975)。但是,器官培养的肝癌迄今未见报道。我们采用二乙基亚硝胺诱发的大鼠移植性的甲胎蛋白呈阳性的肝癌(BERH-2)(强家模等,1978),以琼脂半固体培基进行器官培养。一组培养物已在体外繁殖并 The organ culture of cancer tissue can provide a more ideal model for the research of anticancer drug screening, tumor cell biological characteristics and tumor cell reverse differentiation. Studies in this area have been reported abroad (Tchao et al., 1968; Ellison et al., 1969; Yarnell and Ambrose, 1969a, b; Rubinstein et al., 1973; Wolff and Wolff 1975). However, organ-cultured liver cancer has not been reported so far. We used diethylnitrosamine-induced rat transplanted alpha-fetoprotein-positive hepatocellular carcinoma (BERH-2) (Qiang et al., 1978) and organ culture on agar semi-solid culture medium. A group of cultures have been bred in vitro