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1982—1983年,作者等对从新疆哈密瓜上分离出的两种病毒西瓜花叶病毒2号(简称WMV—2)和黄瓜花叶病毒(简称CMV)进行了提纯,制备了抗血清。用部分提纯的CMV注射兔子,获得了1/512效价的抗血清。用制备的抗血清与CMV—3(黄瓜株系)和CMV—4(豇豆株系)进行了琼质双扩散,发现新疆哈密瓜上的CMV与CMV—3和CMV—4有一定相关。WMV—2提纯比较困难,因为在提纯过程中病毒极易聚集。用PEG沉淀法病毒重新悬浮困难,丢失较多,最后采用差速离心。在超速离心对加20％蔗糖垫。在两次差速离心后再经一次20—(?)%蔗糖梯度离心,得到了较好的提纯效果,制备了1/64效价的抗血清。用作者等制备的WMV—2抗血清与美国、丹麦和日本的WMV抗血清进行了比较试验,证实新疆的WMV—2与美国和丹麦的WMV—2有相同的抗原性。用作者等制备的WMV—2抗血清与哈密瓜病株的蔓、叶、花、果及种子进行血清反应,发现病株的蔓、叶、花、果各部均有大量病毒存在。
In 1982-1983, the authors prepared antisera by purifying the two viruses, watermelon mosaic virus 2 (WMV-2) and cucumber mosaic virus (CMV), isolated from the Hami melon in Xinjiang. Rabbits were injected with partially purified CMV to obtain antiserum at 1/512 titer. The antiserum against CMV-3 (cucumber line) and CMV-4 (cowpea line) were used to perform double QD diffusion. It was found that there was a correlation between CMV and CMV-3 and CMV-4 in Xinjiang Hami melon. WMV-2 purification more difficult, because the virus in the purification process can easily gather. With PEG precipitation virus resuspended difficult, lost more, and finally the use of differential centrifugation. Spent in ultracentrifugation plus 20% sucrose pad. After two differential centrifugations and a 20 - (?)% Sucrose gradient centrifugation, a better purification effect was obtained and a 1/64 titers antisera were prepared. The WMV-2 antiserum prepared by the author and the like were compared with WMV antisera in the United States, Denmark and Japan, and confirmed that WMV-2 in Xinjiang has the same antigenicity as WMV-2 in the United States and Denmark. Serum reaction of the WMV-2 antiserum prepared by the author and the vine, leaf, flower, fruit and seeds of the melon diseased plants showed that a large number of viruses existed in the branches, leaves, flowers and fruits of the diseased plants.