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本文采用超声破碎ＴｒｉｔｏｎＸ—１００和超速离心技术提取了鼠伤寒杆菌（Ｓａｌｍｏｎｅｌｌａｔｙｐｈｉｍｕｒｉｕｍ，ＳＴＭ）的外膜蛋白（Ｏｕｔｅｒｍｅｍｂｒａｎｅｐｒｏｔｅｉｎｓ，ＯＭＰｓ），并发现ＯＭＰｓ中脂多糖ＬＰＳ的含量约为５％，ＯＭＰｓ经ＳＤＳ—ＰＡＧＥ显示１０余条蛋白带。对ＯＭＰｓ诱发ＢＡＬＢ／Ｃ小鼠产生典型迟发型变态反应ＤＴＨ和ＩＬ—２的水平进行了检测。经腹腔免疫的小鼠用５００ＬＤ５０鼠伤寒杆菌（５０１１５）攻击，１００％可得到保护；用５００ＬＤ５０伤寒杆菌（Ｅ６８６）攻击，３３．３％可得到交叉保护。免疫ＢＡＬＢ／Ｃ小鼠的Ｔ淋巴细胞，经尾静脉注射给非免疫小鼠，可使后者得到８５．７％的被动免疫保护，上述结果说明ＯＭＰｓ能诱发ＢＡＬＢ／Ｃ小鼠细胞免疫和保护性免疫，并提示成为分子疫苗的可能性。
In this paper, Outermembrane proteins (OMPs) of Salmonella typhimurium (STM) were extracted by ultrasonic disruption TritonX-100 and ultracentrifugation techniques. The content of lipopolysaccharide LPS in OMPs was about 5% Show more than 10 protein bands. The levels of DTH and IL-2 in typical OMPs-induced delayed-type hypersensitivity in BALB / c mice were detected. The mice immunized intraperitoneally were challenged with 500 LD50 Salmonella typhimurium (50115) and 100% protected; challenge with 500 LD50 Salmonella typhi (E686) resulted in cross-protection of 33.3%. Immunization of BALB / C mice with T lymphocytes via tail vein injection of non-immunized mice resulted in 85.7% passive immunization of the latter, indicating that OMPs induce cellular immunity and protection in BALB / C mice Immunity, and prompts the possibility of becoming a molecular vaccine.