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SBP是一类植物特有的转录因子,在调节花发育、信号转导和抗逆反应中均具有重要作用。为研究毛竹(Phyllostachys edulis)中SBP的分子特征和表达模式,开展了全基因组序列分析。结果表明,毛竹含有32个基因编码SBP转录因子,依次命名为PeSBP1~PeSBP32,各基因的结构差异明显,外显子数量2~11个不等,其中15个SBP基因具有mi R156的结合位点。蛋白结构分析显示,PeSBPs具有2个典型的保守锌指结构域结合位点,且具有核定位信号。系统进化分析表明,32个PeSBPs隶属于8个亚类,且与水稻的亲缘关系最近。表达模式分析表明,32个PeSBPs在叶、鞭、根、20 cm笋、50 cm笋、早花期花序和晚花期花序等7个组织中的表达差异明显,呈现出不同的表达模式。本研究为深入研究毛竹SBP转录因子家族的功能提供了参考依据。
SBP is a plant-specific transcription factor that plays an important role in regulating floral development, signal transduction and anti-retrogradation reactions. To study the molecular characteristics and expression patterns of SBP in Phyllostachys edulis, genome-wide sequence analysis was performed. The results showed that Phyllostachys pubescens contained 32 genes encoding SBP transcription factor, named as PeSBP1 ~ PeSBP32 in turn. The structural differences of each gene were obvious, ranging from 2 to 11 exons, of which 15 SBP genes had mi R156 binding sites . Protein structure analysis showed that PeSBPs has two typical zinc finger domain binding sites with nuclear localization signal. Phylogenetic analysis showed that 32 PeSBPs belonged to 8 subgroups and their genetic relationship with rice was the closest. The expression patterns of 32 PeSBPs showed obvious differences in 7 tissues including leaves, whip, roots, 20 cm bamboo shoots, 50 cm bamboo shoots, early flowering inflorescences and late flowering inflorescences, showing different expression patterns. This study provides a reference for further study on the function of SBP transcription factor family in Phyllostachys pubescens.