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以聚乙烯吡咯烷酮(PVP)为添加剂对毛细管柱进行动态修饰,用于碱性蛋白分离。对4种碱性蛋白质进行分离,实验结果表明聚乙烯吡咯烷酮能够很好地抑制电渗流(EOF)及碱性蛋白在石英毛细管壁上的吸附作用。在pH4.0,PVP浓度(W/V)为0.4％时,EOF仅为1.35×10~(-9) m~2/V.s,平均柱效可达5×10~5理论塔板数/m。每次运行之间(n=6),天与天之间(n=6),迁移时间的相对标准偏差(RSD％)分别小于1％和3％,表明该动态涂敷方法具有良好的重现性和稳定性。另外,由于PVP的粘度较小,使得该方法操作方便、快捷。
Polyvinylpyrrolidone (PVP) as an additive to the capillary column was dynamically modified for alkaline protein separation. The results showed that polyvinylpyrrolidone can inhibit the adsorption of EOF and basic protein on the capillary walls of quartz. At pH 4.0, the EOF was only 1.35 × 10 -9 m 2 / Vs with PVP concentration (W / V) of 0.4% and the average column efficiency was 5 × 10 ~ 5. The number of theoretical plates / m . The relative standard deviation (RSD%) of migration times was less than 1% and 3% respectively between each run (n = 6) and between days and days (n = 6), indicating that the dynamic coating method had good weight Reality and stability. In addition, due to the smaller viscosity of PVP, the method is convenient and fast to operate.