目的　检测室内高氡暴露地区居民肺癌p5 3和k ras基因突变。方法　采用PCR扩增、PCR SSCP电泳和序列分析方法。结果　 9例标本中有 5例p5 3PCR SSCP电泳呈阳性结果 ,其中 3例经克隆测序发现有碱基置换。结论　室内氡浓度在 2 0 0～ 35 0Bq·m- 3·a- 1 范围内的 7例肺癌病例中有 5例发生p5 3基因突变 ,p5 3基因突变还可能与肺癌患者的年龄及吸烟史有关。 Objective To detect p53 and k ras mutations in lung cancer in residents exposed to indoor sorghum. Methods PCR amplification, PCR SSCP electrophoresis and sequence analysis methods were used. Results There were 5 cases of p5 3PCR SSCP electrophoresis positive results in 9 specimens, of which 3 cases were found to have base substitutions by cloning and sequencing. Conclusion There were 5 cases of p53 gene mutations in 7 lung cancer cases with indoor radon concentrations ranging from 200 to 35 0 Bq·m -3·a - 1. The p53 gene mutation may also be related to the age and smoking history of lung cancer patients. related.