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Objective:To evaluate the effects of HPVI6 E6/E7 siRNAs on cervical cancer SiHa cells.Methods:The expressions of the E6,E7,p53 and Rb genes were assayed by RT-PCR and Western-bloting respectively.The proliferation and apoptosis of the cells were evaluated by MTT and flow cytometry.Results:HPV 16 E6 and E7 oncogenes were selectivly downregulated by HPV 16 E6 and E7 siRNAs,which sustained at least 96 h by single dose siRNA.Furthermore,reduction of E6 and E7 oncogenes expression upregulated the expressions of P53 and RB protein and induced apoptosis in SiHa cells.Conclusion: Introduction of HPV16 E6/E7 siRNA might be a potentially potent and specific approach to inhibit proliferation and induce apoptosis of SiHa cervical cancer cells.
Objective: To evaluate the effects of HPVI6 E6 / E7 siRNAs on cervical cancer SiHa cells. Methods: The expressions of the E6, E7, p53 and Rb genes were assayed by RT-PCR and Western-blotting respectively. The proliferation and apoptosis of the cells were evaluated by MTT and flow cytometry. Results: HPV 16 E6 and E7 oncogenes were selectivly downregulated by HPV 16 E6 and E7 siRNAs, which sustained at least 96 h by single dose siRNA. Future and reduction of E6 and E7 oncogenes expression upregulated the expressions of P53 and RB protein and induced apoptosis in SiHa cells. Contact: Introduction of HPV16 E6 / E7 siRNA might be a potentially potent and specific approach to inhibit proliferation and induced apoptosis of SiHa cervical cancer cells.