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以陆地棉“泗棉３号”、“北无２０３１”、“鲁棉９５５４”为材料进行全固体体细胞培养，获得了胚性愈伤组织和再生植株。起始愈伤组织阶段，用含２，４－Ｄ０．１ｍｇ／Ｌ、ＫＴ０．１ｍｇ／Ｌ和２，４－Ｄ０．１ｍｇ／Ｌ、ＩＡＡ０．１ｍｇ／Ｌ、ＺＴ０．１ｍｇ／Ｌ的培养基效果较好；进入胚性愈伤组织诱导阶段，在含ＩＡＡ０．５ｍｇ／Ｌ、ＫＴ０．５ｍｇ／Ｌ的培养基上胚性愈伤组织诱导频率较高。使用ＳＨ培养基或将ＭＳ培养基ＮＨ４ＮＯ３的浓度降为０．３ｇ／Ｌ，可大幅度提高胚性愈伤组织诱导频率。在胚状体萌发培养基中添加２５０ｍｇ／Ｌ活性炭，可减轻胚状体褐化率，提高成苗率。对于在萌发培养基中未能长出真叶的胚状体，可将其转至添加ＩＢＡ０．４ｍｇ／Ｌ、ＫＴ０．１ｍｇ／Ｌ的培养基，部分胚状体能培养成苗。
All solid-state cell culture was performed on upland cotton “Simian 3”, “North No 2031” and “Lumi 9554”. Embryogenic callus and regenerated plants were obtained. The initial callus stage, with 2,4-D0.1mg / L, KT0.1mg / L and 2,4-D0.1mg / L, IAA0.1mg / L, ZT0.1mg / L medium effect Better; into the embryogenic callus induction stage, with IAA0.5mg / L, KT0.5mg / L medium embryogenic callus induction frequency higher. Using SH medium or reducing the concentration of NH4NO3 in MS medium to 0.3g / L, the frequency of embryogenic callus induction can be greatly improved. Adding 250mg / L activated carbon into embryoid germination medium can reduce the embryoid body browning rate and improve the seedling formation rate. For embryoid bodies that failed to grow true leaves in germination medium, they were transferred to a medium supplemented with 0.4 mg / L IBA and 0.1 mg / L KT, and some embryoids could be cultured into seedlings.