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目的制备一种新型的硫醇修饰的磁性银花纳米粒子SERS基底对氯霉素(CAP)进行拉曼检测,确定SERS基底的增强效果。方法选择烷基硫醇作为基底的表面修饰剂,自组装到磁性银花纳米粒子的银壳表面,通过疏水作用,将CAP分子富集到基底表面,从而实现检测CAP分子拉曼信号的增强。结果 3种不同硫醇修饰的基底对CAP的增强顺序是正己硫醇>十二硫醇>十八硫醇。利用Fe_3O_4@SiO_2-Ag-C6 SERS基底对浓度范围10~(-3)~10~(-10)mol/L的CAP溶液和10~(-3)~10~(-9)mol/L的牛奶中CAP进行拉曼检测,检测限分别为0.1 nmol/L(32 ppt)和1 nmol/L(323 ppt)。结论硫醇修饰的磁性银花纳米粒子是一种高活性的SERS基底,可用于低浓度物质的检测分析。
Objective To prepare a novel thiol-modified magnetic silver flower nanoparticle SERS substrate for the detection of chloramphenicol (CAP) by Raman to determine the enhancement effect of SERS substrate. Methods Alkylthiol was chosen as the surface modifier of the substrate to self-assemble into the surface of silver shell of magnetic silver flower nanoparticles. CAP molecules were enriched onto the surface of the substrate by hydrophobic interaction, so as to detect Raman signal enhancement of CAP molecule. Results The enhancement order of CAP for three different thiol-modified substrates was n-hexanethiol> dodecyl mercaptan> octadecanethiol. The concentration of CAP solution of 10 ~ (-3) -10 ~ (-10) mol / L and the concentration of 10 ~ (-3) ~ 10 ~ (-9) mol / L in the solution of Fe_3O_4 @ SiO_2-Ag- CAP in milk was detected by Raman, with detection limits of 0.1 nmol / L (32 ppt) and 1 nmol / L (323 ppt), respectively. Conclusion The mercaptan modified magnetic silver nanoparticles is a highly active SERS substrate, which can be used for the detection and analysis of low concentration of substances.