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由肠道病毒第70型引起急性出血性结膜炎的治疗药物问题,以往在临床观察和实验室药物筛选中均未获得满意结果。木文报道取100TCTD50/0.1毫升病毒与不同浓度的各种药物等量混和,置37℃1小时,然后接种于传代的人胚肺细胞,观察药物对细胞病变的抑制情况。用此方法对50多种抗病毒的化合物及中草药进行了筛选。实验结果,槲寄生的提取物(由上海医药工业研究院提供)和桂枝(由本所提供)两种中药分别对本病毒有一定的抑制作用。提取物的最小抑制浓度为0.156～0.313毫克(相当于槲寄生生药31.2～62.5毫克):桂枝的最小抑制浓度以生药含量计50毫克。另外,我们进一步用不同浓度的槲寄生提取物药液,将之先加入细胞管,2小时或24小时后再接种病毒,或先接种病毒于细胞管2小时后再加入含不同浓度药液的维持液等实验方法,三者均取得相同的抑制效果。但是如将经1小时处理的病毒药物混合液接种细胞管后2小时
The treatment of acute hemorrhagic conjunctivitis caused by enterovirus type 70 has not been satisfactorily achieved in both clinical observation and laboratory drug screening. Wood reported 100TCTD50 / 0.1 ml virus and different concentrations of various drugs equal mixing, set at 37 ℃ for 1 hour, and then inoculated on passage of human embryonic lung cells observed drug inhibition of cytopathic effect. More than 50 antiviral compounds and Chinese herbal medicines were screened by this method. The experimental results, mistletoe extract (by the Shanghai Institute of Pharmaceutical Industry) and Guizhi (provided by the two kinds of traditional Chinese medicine, respectively, the virus has some inhibitory effect. The minimum inhibitory concentration of the extract was 0.156 ~ 0.313 mg (equivalent to 31.2 ~ 62.5 mg of mistletoe crude drug): The minimum inhibitory concentration of Cassia twig was 50 mg based on crude drug content. In addition, we further use different concentrations of mistletoe extract solution, the first added to the cell tube, 2 hours or 24 hours after the virus inoculation, or the first virus in the cell tube 2 hours before adding different concentrations of liquid Maintenance of liquid and other experimental methods, all three have achieved the same inhibitory effect. However, 2 hours after inoculation of the cell tube with the virus drug mixture to be treated for 1 hour