论文部分内容阅读
为确定一起杂交鲇皮肤溃疡症的病原,实验从病鱼体内分离到几株优势菌(DYJ140914-DYJ140917),根据4株分离菌的形态、生理生化特性,结合16S rRNA和gyrB基因序列测定(Gen Bank登录号分别为KP693689和KP693690)与系统发育分析,将其鉴定为恶臭假单胞菌(Pseudomonas putida)。在此基础上以腹腔注射的方式进行人工感染试验,证实其为杂交鲇溃疡症的病原菌。病鱼组织器官具有典型的病理变化,其主要靶器官为肝脏、皮肤肌肉以及肾间质,分别引起多灶性坏死性肝炎、坏死性肌炎及坏死性间质性肾炎。此外,还可引起心外膜、心内膜炎及坏死性脾炎。药敏结果显示该菌对强力霉素、诺氟沙星和左氧氟沙星等药物敏感;对青霉素、氟苯尼考、磺胺甲基异恶唑、头孢西丁、阿奇霉素等药物耐药。
In order to determine the pathogen of skin ulcer with hybridization, several dominant bacteria (DYJ140914-DYJ140917) were isolated from diseased fish. According to the morphological, physiological and biochemical characteristics of the four isolates, the 16S rRNA and gyrB gene sequences were determined Bank accession numbers KP693689 and KP693690 respectively) and phylogenetic analysis, which was identified as Pseudomonas putida. On this basis, intraperitoneal injection of artificial infection test confirmed that it is the pathogen of hybrid ulcer disease. Diseased fish tissues and organs have typical pathological changes, the main target organ for the liver, skin and muscle interstitium, respectively, caused by multifocal necrotizing hepatitis, necrotizing myositis and necrotizing interstitial nephritis. In addition, can also cause epicardium, endocarditis and necrotizing spleen. Susceptibility results showed that the strain was sensitive to drugs such as doxycycline, norfloxacin and levofloxacin, and resistant to penicillin, florfenicol, sulfamethoxazole, cefoxitin and azithromycin.