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采用单甲基醚聚乙二醇(mPEG-OH)接枝支化聚乙烯亚胺(hy-PEI),得到水溶性共聚物聚乙二醇接枝聚乙烯亚胺(mPEG-g-PEI),通过配体交换的方法包裹超顺磁性四氧化三铁纳米粒子(SPIO),再通过功能化的聚乙二醇(mal-PEG-COOH)与活化的前列腺干细胞抗原(PSCA)单克隆单链抗体结合,获得前列腺癌细胞靶向核磁共振显像纳米探针.研究表明,负载超顺磁性四氧化三铁纳米粒子的聚乙二醇接枝聚乙烯亚胺(mPEI-g-PEG-SPIO)的粒径约为50nm,能显著提高对前列腺癌细胞的特异性输送效率,从而显著地降低前列腺癌细胞的核磁共振成像(MRI)T2信号强度,增强对前列腺癌的识别效率,有可能成为一种前列腺癌MRI早期诊断新型纳米探针或者基因治疗中一种MRI可见的核酸输送载体.
Polyethylene glycol grafted polyethyleneimine (mPEG-g-PEI) was obtained by grafting branched polyethyleneimine (hy-PEI) with monomethyl ether polyethylene glycol (mPEG-OH) , Superparamagnetic iron tetroxide nanoparticles (SPIO) were encapsulated by ligand exchange method, and then purified by functionalized polyethylene glycol (mal-PEG-COOH) and Activated Prostate Stem Cell Antigen Antibody to obtain prostate cancer cell targeting NMR imaging probe.The results show that polyethylene glycol grafted polyethyleneimine (mPEI-g-PEG-SPIO) loaded with superparamagnetic iron tetroxide nanoparticles, Particle size of about 50nm, can significantly improve the specific delivery efficiency of prostate cancer cells, thereby significantly reducing prostate cancer cells MRI T2 signal intensity, and enhance the recognition efficiency of prostate cancer, it may become a Prostate Cancer MRI Early Diagnosis of Novel Nanoprobes or Gene Therapy A MRI-Accessible Nucleic Acid Delivery Vehicle.