作者采用 O-dianisdine 比色法测定粒细胞过氧化酶(myeloperoxidase,MPO)活性对鼠大脑中动脉阻塞模型缺血灶内白细胞浸润情况进行研究。选用250～300g 雄性豚鼠,麻醉后固定于定位脑手术台上,开颅避开嗅索暴露左侧大脑中动脉,用双极电凝夹分别阻断该处血流,于1,2,4,6,8,16,24,48,96h 内断头采样取缺血灶内3个部位,对侧相应处 The authors used O-dianisdine colorimetric assay of myeloperoxidase (MPO) activity of white blood cells within the ischemic lesions of the middle cerebral artery occlusion model in rats. Selection of 250 ~ 300g male guinea pigs, anesthesia fixed in the positioning of the brain surgery table, craniotomy to avoid the olfactory cord exposed left middle cerebral artery, bipolar coagulation clamp were used to block the blood flow in the 1,2,4 , 6,8,16,24,48,96 h within the decapitation sampling take three sites within the ischemic lesion, contralateral counterparts