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目的 研究洛沙坦对糖尿病大鼠肾组织一氧化氮（NO）水平的影响。方法 雄性Wistar大鼠分为3组,A组（11只）为正常对照组,B组（11只）为糖尿病未干预组,C组（9只）为糖尿病大鼠洛沙坦干预组。以链脲菌素制备糖尿病大鼠模型,大鼠饲养18周后取出肾脏检测诱导型NO合成酶（iNOS）mRNA的表达,电镜检测大鼠肾小球基底膜厚度及系膜基质密度（系膜基质面积/系膜面积）。收集24h尿测定尿白蛋白排泄（UAE）及肌酐,并心脏内取血检测血肌酐。mRNA表达采用RT-PCR,以β-actin作为内对照。UAE测定采用大鼠白蛋白特异的酶免疫分析试剂盒。结果 肾组织iNOSmRNA表达在B组大鼠（0.30±0.12）显著高于A组（0.12±0.04,P<0.01）,C组（0.25±0.14）与B组比较差异无显著性意义（P>0.05）。肾组织NO水平在B组大鼠[（0.56±0.20）μmol/mg肾组织]显著低于A组[（1.05±0.25）μmol/mg肾组织]和C组[（1.13±0.62）μmol/mg肾组织,P均<0.01]。UAE在B组大鼠[（2.18±1.98）mg/d]显著高于A组[（0.41±0.47）mg/d]和C组[（0.65±0.89）mg/d,P均<0.05]。肌酥清除率在B组大鼠[（19.75±9.60）ml/d]显著低于A组[（59.63±22.75）ml/d]和C组[（40.88±25.57）ml/d,P均<0.05]。基底膜厚度在B组大鼠[（531.6±107.6）nm]显著高于A组[（312.4±25
Objective To investigate the effect of losartan on the level of nitric oxide (NO) in kidney of diabetic rats. Methods Male Wistar rats were divided into 3 groups: group A (n = 11) as normal control group, group B (n = 11) as diabetic intervention group and group C (n = 9) as losartan intervention group. The diabetic rat model was established by streptozotocin. After 18 weeks of rearing, the kidneys were removed and the expression of iNOS mRNA was detected. The glomerular basement membrane thickness and mesangial matrix density Matrix area / mesangial area). Urine albumin excretion (UAE) and creatinine were collected 24h urinalysis, and serum creatinine was measured in the heart. The mRNA expression was determined by RT-PCR and β-actin as the internal control. UAE assay using rat albumin-specific enzyme immunoassay kit. Results The expression of iNOS mRNA in renal tissue was significantly higher in group B than that in group A (0.12 ± 0.04, P <0.01), while there was no significant difference in group C (0.25 ± 0.14) and group B (P> 0.05 ). The level of NO in kidney in group B was significantly lower than that in group A [(1.06 ± 0.20) μmol / mg kidney [(1.05 ± 0.25) μmol / mg kidney and group C [1.13 ± 0.62 μmol / Kidney, P <0.01]. UAE in group B was significantly higher than that in group A [(2.18 ± 1.98) mg / d] [(0.41 ± 0.47) mg / d] and group C [(0.65 ± 0.89) mg / d, P <0.05; Muscle crisp clearance rate in group B was significantly lower than that in group A [(19.75 ± 9.60) ml / d and [59.63 ± 22.75] ml / d and 40.88 ± 25.57 ml / d, respectively, P < 0.05]. Basement membrane thickness in group B [(531.6 ± 107.6) nm] was significantly higher than that in group A [(312.4 ± 25