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研究运用蓝细菌和硅藻16SrDNA特异引物,将晚季水稻生长后期稻田土壤中提取的总DNA进行PCR扩增后,以DGGE技术对PCR产物进行分析结果表明,14条DGGE带经克隆测序,经NCBI基因库比对得晚季水稻生长后期存在10种蓝细菌,包括4种Leptolyngbya、1种Chamaesiphon、1种Nostoc、1种Oscillatoria、2种Syne-chococcus和1种Chroococcidiopsis。同层不同位置土壤中蓝细菌种群亦有所不同,但每个取样点都有一些特有的蓝细菌种类。用常规方法对同一稻田土壤样品进行分离培养,根据蓝细菌鉴定图谱观察到类似Lyngbya、Oscillatori-a、Chroococcidiopsis及Nostoc的蓝细菌,但显微镜下无法准确分类。比较结果表明采用DGGE法比常规培养法能更准确进行蓝细菌多态性鉴定。
In the present study, the total DNA extracted from paddy soils in late rice growth stage was amplified by PCR using 16SrDNA specific primers from cyanobacteria and diatoms. The PCR products were analyzed by DGGE. The results showed that 14 DGGE bands were cloned and sequenced. There are 10 species of cyanobacteria in NCBI gene late rice growing stage, including 4 Leptolyngbya, 1 Chamaesiphon, 1 Nostoc, 1 Oscillatoria, 2 Synechococcus and 1 Chroococcidiopsis. The cyanobacteria populations are also different in soils of the same layer, but there are some species of cyanobacteria unique to each sampling site. The same paddy soil samples were isolated and cultured by conventional methods. According to the identification of cyanobacteria, cyanobacteria such as Lyngbya, Oscillatori-a, Chroococcidiopsis and Nostoc were observed, but they could not be accurately classified under the microscope. The results showed that the DGGE method could identify the cyanobacteria more accurately than the conventional culture method.