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目的对比研究羟丁酸钠和氯胺酮对原代培养大鼠心肌细胞的毒性作用。方法将经原代培养成活4d后的大鼠心肌细胞分为5组 ,每组6孔。包括对照组 ,小剂量(HL ,3×10-3mol·L -1)与大剂量(HH ,3×10 -2mol·L -1)羟丁酸钠组和小剂量(KL ,1×10 -5mol·L -1)与大剂量(KH ,1×10-4 mol·L-1)氯胺酮组。各组均于实验开始后8h终止反应 ,评定心肌细胞搏动功能、观察细胞形态学改变、测定心肌细胞酶及电解质变化。结果与对照组相比 ,KL组心肌细胞搏动频率明显加快(P<0.05) ,而KH组减慢(P<0.05)。细胞形态学亦有相应变化。KH组LDH和AST释放量增加(P<0.05) ,ALP活性下降(P<0.05)。而HL、HH组的上述指标均无明显变化。各组间电解质变化未见显著差异。结论高浓度的氯胺酮具有直接的心肌抑制作用 ,而低浓度的氯胺酮却有正性变时性和变力性作用。无论低浓度或高浓度的羟丁酸钠 ,均未见心肌细胞毒性作用。
Objective To compare the toxic effects of sodium oxybate and ketamine on primary cultured rat cardiomyocytes. Methods The primary cultured cardiomyocytes were divided into 5 groups, 6 holes in each group. Including the control group, small dose (HL, 3 × 10-3mol·L -1) and high dose (HH, 3 × 10-2mol·L -1) sodium oxybate group and low dose (KL, 1 × 10 - 5mol·L -1) and high dose (KH, 1 × 10-4 mol·L -1) ketamine group. Each group was terminated at 8h after the start of the experiment, the cardiomyocyte cardiomyocyte function was assessed, morphological changes of the cells were observed, and enzyme and electrolyte changes of cardiomyocytes were measured. Results Compared with the control group, the frequency of cardiomyocytes in KL group was significantly increased (P <0.05), but decreased in KH group (P <0.05). Cell morphology also has corresponding changes. KH group LDH and AST release increased (P <0.05), ALP activity decreased (P <0.05). The HL, HH group of the above indicators did not change significantly. Electrolytes between groups showed no significant difference. Conclusions Ketamine at high concentration has a direct myocardial inhibition, whereas ketamine at low concentrations has positive chronotropic and inotropic effects. Neither low or high concentrations of sodium oxybate showed cardiomyocyte cytotoxicity.