论文部分内容阅读
目的建立博尔纳病病毒(Borna disease virus,BDV)磷蛋白的细胞模型,并对所建模型进行鉴定。方法重新扩增和鉴定已有的BDV磷蛋白(GFP-P24)质粒,使用转染试剂将该质粒转入PC12细胞,并用荧光定量PCR和ELISA的方法对所构建的细胞模型进行鉴定。结果重新扩增的质粒PCR鉴定阳性,其浓度符合转染的需要,测序后未发现核苷酸的突变;转染PC12细胞的效率高,荧光定量PCR和ELISA检测均为阳性。结论成功构建了BDV磷蛋白的细胞模型,为研究BDV感染过程中磷蛋白所起的作用奠定了基础。
Objective To establish a cell model of Borna disease virus (BDV) phosphoprotein and to identify the model. Methods The existing plasmid of BDV phosphoprotein (GFP-P24) was amplified and identified. The plasmid was transfected into PC12 cells using transfection reagent. The constructed cell model was identified by fluorescence quantitative PCR and ELISA. Results The PCR products of the re-amplified plasmids were positive and their concentrations were in accordance with the requirements of transfection. No nucleotide mutation was found after sequencing. The transfection efficiency of PC12 cells was high, and both of them were positive by fluorescence quantitative PCR and ELISA. Conclusion The cell model of BDV phosphoprotein was successfully constructed, which lays the foundation for studying the role of phosphoprotein during BDV infection.