目的：研究癌细胞的内质网膜系统与其侵袭性之间的相互关系。方法：应用共焦激光扫描显微技术观察不同侵袭力的人大肠癌细胞 Ｃ Ｃ Ｌ２２９ 及 Ｃ Ｘ１ 的内质网膜系统，结合流式细胞仪技术测定两者内质网膜系统的荧光强度。结果：两种癌细胞内均存在着立体网络状结构的内质网膜系统，细胞核周围致密，周边逐渐稀疏。高侵袭力的 Ｃ Ｃ Ｌ２２９ 细胞的内质网发达，伪足中存在独枝或立体网络状的内质网，而低侵袭力的 Ｃ Ｘ１ 细胞的内质网相对不发达，未见伪足样结构。流式细胞仪检测 Ｃ Ｃ Ｌ２２９ 细胞的 Ｅ Ｒ 的平均荧光强度为１ ０２４ ±５０２９７ ， Ｃ Ｘ１ 细胞的 Ｅ Ｒ 的平均荧光强度为４６２９０ ±２１７１８ ，统计学处理两者差异显著（ Ｐ＜ ００１） 。结论：高侵袭力的 Ｃ Ｃ Ｌ２２９ 细胞与低侵袭力的 Ｃ Ｘ１ 细胞的内质网膜系统形态及数量上均存在差异，可能与内质网合成和侵袭相关之分泌蛋白的功能有关。 Objective: To study the relationship between the endoplasmic reticulum of cancer cells and their invasiveness. METHODS: Confocal laser scanning microscopy was used to observe the endoplasmic reticulum system of human colorectal cancer cells C C L 229 and C X  1 with different invasiveness, and the fluorescence of both endoplasmic reticulum systems was measured by flow cytometry. strength. RESULTS: There was a three-dimensional network-like endoplasmic reticulum system in both cancer cells. The nucleus was dense and the periphery was gradually sparse. The high invasiveness of C C L229 cells resulted in the development of the endoplasmic reticulum, and the presence of a single or three-dimensional network of endoplasmic reticulum in the pseudopodia, whereas the low invasiveness of the C X1 cell endoplasmic reticulum was relatively undeveloped. Foot-like structure. The mean fluorescence intensity of E R in C C L229 cells was 1 024 ±50297 by flow cytometry, and the average fluorescence intensity of E R in C X1 cells was 46290 ±21718. The difference was significant (P<001). CONCLUSION: The high invasiveness of C C L 229 cells and the low invasiveness of C X  1 cells have differences in the morphology and number of endoplasmic reticulum membranes, which may be related to the function of secretory proteins involved in endoplasmic reticulum synthesis and invasion.