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通过不同浓度的低密度脂蛋白与体外培养的新生小牛主动脉内皮细胞经不同时间孵育后，应用凝血酶、蛋白Ｃ和Ｓ－２２３８发色底物显色法，观察单层内皮细胞膜上血栓调节蛋白功能的变化。结果显示：低密度脂蛋白孵育浓度＞６ｍｇ·Ｌ－１时．血栓调节蛋白活性降低，两者呈良好的负相关（ｙ＝－０．２５ｘ＋６５．９６，ｒ＝－０．８７，ｎ＝１７５，Ｐ＜０．００１）；浓度≥５０ｍｇ·Ｌ－１时，随着孵育时间延长，血栓调节蛋白受抑制较显著，并呈相关性；低密度脂蛋白还使血栓调节蛋白辅助激活蛋白ｃ的反应速率出现早、中期减慢，后期加快的变化．这些变化表明低密脂蛋白具有形响血栓调节蛋白功能的作用，提示高脂血症可以通过抑制动脉血管壁局部抗凝功能来促进动脉粥样硬化的发生发展．
By different concentrations of low density lipoprotein and in vitro cultured neonatal bovine aortic endothelial cells incubated at different times, the thrombin, protein C and S-2238 chromogenic substrate colorimetric method was used to observe the monolayer endothelial cell membrane thrombus Regulates protein function changes. The results showed that when the concentration of LDL was> 6 mg · L-1. The thrombomodulin activity decreased, with a good negative correlation (y = -0.25x + 65.96, r = -0.87, n = 175, P <0.001) With the prolongation of incubation time, thrombomodulin was significantly inhibited and correlated. Low density lipoprotein also caused the response rate of thrombomodulin-activated protein c to decrease in the early, middle and late stages. These changes indicate that lipoproteins have the function of thrombomodulin, suggesting that hyperlipidemia can promote the development of atherosclerosis by inhibiting the local anticoagulant function of the arterial wall.