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目的探讨负载磺胺嘧啶银(silver sulfadiazine,AgSD)纳米片对轻度烧伤合并耐甲氧西林金黄色葡萄球菌小鼠的治疗效果。方法利用纳米技术制备负载0.1、1.0、10.0、20.0、50.0、100.0μg/cm~2 AgSD颗粒纳米片,应用原子力显微镜观察其形态特征,确定最佳治疗浓度。雄性C57BL6小鼠18只腹腔麻醉后将背部毛发除去,暴露于70℃热水中4s,制备小鼠烧伤模型,1d后在伤口处接种10u耐甲氧西林金黄色葡萄球菌,制备浅度烧伤并耐甲氧西林金黄色葡萄球菌感染模型,并随机分为观察组、治疗对照组、空白对照组各6只。观察组应用负载20.0μg/cm~2 AgSD纳米片进行治疗,治疗对照组应用未负载AgSD的纳米片进行治疗,空白对照组不进行任何治疗,治疗3d后测量3组伤口面积,统计伤口细菌数,显微镜观察烧伤组织病理改变。结果随AgSD浓度增高,纳米片形成的AgSD颗粒增大,AgSD颗粒和纳米片的黏附力降低;确定纳米片负载AgSD的最佳浓度为20μg/cm~2;治疗3d后,观察组伤口感染面积[(25.1±0.9)cm~2]较治疗对照组[(46.3±2.5)cm~2]、空白对照组[(47.1±1.5)cm~2]明显缩小(P<0.01);观察组伤口感染细菌数[(2.1±0.2)×10~2 cfu]较治疗对照组[(1.4±0.2)×10~6 cfu]、[空白对照组(2.5±0.1)×10~6 cfu]明显减少(P<0.01);观察组伤口感染程度较治疗对照组、空白对照组明显减轻(P<0.01)。结论负载AgSD纳米片治疗轻度烧伤合并耐甲氧西林金黄色葡萄球菌感染具有较好的治疗效果。
Objective To investigate the therapeutic effect of silver sulfadiazine (AgSD) loaded nano-tablets on mice with mild burn and methicillin-resistant Staphylococcus aureus. Methods Nanoparticles were used to prepare AgSD nanoparticle loaded with 0.1,1.0,10.0,20.0,50.0,100.0μg / cm ~ 2 AgSD. Morphological characteristics were observed by atomic force microscopy and the best therapeutic concentration was determined. Male C57BL6 mice were anesthetized by intraperitoneal anesthesia, the back hair was removed, exposed to hot water at 70 ℃ for 4s to prepare a mouse model of burn, 1d after the wound was inoculated with 10u methicillin-resistant Staphylococcus aureus, the preparation of shallow burns and Methicillin-resistant Staphylococcus aureus infection model and randomly divided into observation group, treatment control group, blank control group, 6 each. The observation group was treated with 20.0μg / cm ~ 2 AgSD nanosheets. The control group was treated with AgSD-loaded nanosheets, the blank control group without any treatment, the wound area was measured after 3 days of treatment, the number of bacteria The pathological changes of the burn tissue were observed with microscope. Results With the increase of AgSD concentration, AgSD particles formed by nanosheets increased and the adhesion of AgSD particles and nanosheets decreased. The optimal concentration of AgSD loaded on nanosheets was 20μg / cm ~ 2. After 3 days of treatment, the wound infection area [(25.1 ± 0.9) cm ~ 2] was significantly lower than that of the control group [(46.3 ± 2.5) cm ~ 2] and that of the blank control group (47.1 ± 1.5 cm 2) (P <0.01) Compared with the control group [(1.4 ± 0.2) × 10 ~ 6 cfu] and the number of bacteria [(2.1 ± 0.2) × 10 ~ 2 cfu] <0.01). The degree of wound infection in the observation group was significantly lower than that in the control group and the blank control group (P <0.01). Conclusion AgSD loaded nano-tablets for mild burns with methicillin-resistant Staphylococcus aureus infection has a good therapeutic effect.