论文部分内容阅读
机体在正常代谢过程中产生的氧自由基可引起DNA氧化性损伤,损伤率与代谢率有关;环境中许多化学、物理因素也可通过氧自由基介导DNA损伤.DNA氧化性损伤同许多年龄有关的退行性疾病关系密切。机体有多水平抗DNA氧化机制,某些外源物质有类似作用。oh~8dG(8-羟基-2‘-脱氧鸟苷)被证实是一个很好的DNA氧化损伤的生物学标记,HPLC/EC法能有效分析组织DNA和尿中oh~8dG的含量。该方法在遗传毒理学、抗衰老医学、肿瘤病因学等领域有广泛应用前景.
The oxygen free radicals produced by the body during normal metabolism can cause DNA oxidative damage, and the damage rate is related to the metabolic rate. Many chemical and physical factors in the environment can also mediate DNA damage by oxygen free radicals. Oxidative DNA damage is associated with many age Relevant degenerative diseases are closely related. The body has multiple levels of anti-DNA oxidation mechanism, some of the exogenous substances have a similar effect. Oh ~ 8dG (8-hydroxy-2’-deoxyguanosine) proved to be a good biomarker for DNA oxidative damage. The HPLC / EC method can effectively analyze the content of oh ~ 8dG in tissue DNA and urine. The method has wide application prospects in the fields of genotoxicity, anti-aging medicine and tumor etiology.