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目的:观察麦冬不同提取物对过氧化氢诱导的人脐静脉内皮细胞(HUVEC)间黏附分子-1(ICAM-1)和VEGF、Bcl-2表达的影响。方法:体外培养HUVEC,用过氧化氢(H202)制造HUVEC损伤模型。以四甲基偶氮唑蓝(MTT)比色法检测细胞存活数量,用流式细胞仪检测HUVEC表面ICAM-1的表达量;免疫细胞化学方法检测HUVEC的VEGF、Bcl-2的分布情况。结果:模型组较正常对照组细胞增殖活性明显降低(P<0.01)。与模型组相比,经麦冬水提物、正丁醇提取物处理组细胞增殖活性明显增加(P<0.05,P<0.01)。流式细胞仪检测显示正丁醇提取物可降低过氧化氢增加的ICAM-1基因的表达。Bcl-2的表达,模型组明显低于正常对照组,而正丁醇组表达明显高于模型组(P<0.01)。VEGF的表达,模型组明显高于正常对照组,麦冬水提物、正丁醇提取物处理组高于模型组(P<0.05,P<0.01)。结论:麦冬提取物具有抗凋亡、促增殖、降低细胞间黏附分子-1表达的作用,尤以正丁醇提取物效果更为显著。
OBJECTIVE: To observe the effects of different extracts of Radix Ophiopogonis on hydrogen peroxide-induced expression of ICAM-1, VEGF and Bcl-2 in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were cultured in vitro and HUVEC injury models were made by hydrogen peroxide (H202). The cell viability was detected by MTT assay. The expression of ICAM-1 on HUVECs was detected by flow cytometry. The distribution of VEGF and Bcl-2 in HUVECs was detected by immunocytochemistry. Results: Compared with normal control group, the proliferation activity of model group was significantly lower (P <0.01). Compared with the model group, the cell proliferation activity of Ophiopogon japonicus water extract and n-butanol extract treatment group increased significantly (P <0.05, P <0.01). Flow cytometry showed that n-butanol extract decreased the expression of ICAM-1 gene, which was increased by hydrogen peroxide. Bcl-2 expression in model group was significantly lower than that in normal control group, while n-butanol group was significantly higher than model group (P <0.01). VEGF expression in the model group was significantly higher than that of the normal control group, Ophiopogon japonicus water extract and n-butanol extract treatment group was higher than the model group (P <0.05, P <0.01). CONCLUSION: Ophiopogon japonicus extract has anti-apoptotic, pro-proliferative effect and reduces the expression of intercellular adhesion molecule-1, especially n-butanol extract.