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利用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)技术和主成分分析法(principal component analysis,PCA)解析了大庆油田聚驱后油藏的细菌和古菌群落结构组成及分布特征.结果表明,注水井中的细菌主要以好氧的假单胞菌属和不动杆菌属为主,注水井近井地带以兼性厌氧的肠杆菌属为主,各采油井中的细菌则包括陶厄氏菌属、梭菌纲、假单胞菌属、油杆菌属及大量的未培养细菌;各注水井及注水井近井地带检测到的古菌主要是乙酸型产甲烷的甲烷鬃菌,各采油井中古菌则以甲烷微菌属、甲烷螺菌属及甲烷杆菌属等为主.总体上,该聚驱区块从注水井到采油井,细菌优势菌群依次呈好氧细菌-兼性厌氧细菌-严格厌氧细菌分布;古菌的分布受环境因素及微生物代谢产物影响,注水井和采油井中的优势菌群差异显著.
The composition and distribution of bacterial and archaeal communities in the reservoir after flooding in Daqing Oilfield were analyzed by denaturing gradient gel electrophoresis (DGGE) and principal component analysis (PCA) , Water injection wells bacteria mainly aerobic Pseudomonas and Acinetobacter mainly water injection well near the well to facultative anaerobic Enterobacteriaceae mainly oil wells in the bacteria include Tao Bacteria, Clostridium, Pseudomonas, Bacillus, and a large number of uncultured bacteria; each injection wells and injection wells near the well-detected archaea is mainly acetic acid methane methane methane bristles, each Methanobacteria, Methanospirillum and Methanobacterium are the main oil producing wells.In general, the poly flooding block from the injection well to the production well, the bacteria superior bacteria in turn were aerobic bacteria - facultative Anaerobic bacteria - strictly anaerobic bacteria distribution; distribution of archaea by environmental factors and microbial metabolites, water injection wells and production wells in the dominant flora significant difference.