In the present study,we aimed to globally profile the proteins involved in colorectal carcinoma(CRC),in order to find clues to the pathological process of CRC.Pairs of malignant tissues and their adjacent healthy tissues from patients with colorectal cancer were subject to differential proteomics analysis.Two dimensional electrophoresis coupled with mass spectrometry(2-DE/MS) was used to identify differentially expressed proteins between pairs of tissue samples.A list of proteins relevant to the progression of colorectal tumor was identified by two dimensional gel electrophoresis(2-DE)-based proteomics approach.Among the identified proteins,vinculin was found to be remarkably down-regulated in colorectal carcinoma tissues.In addition,three phosphorylation modifications within the isolated vinculin were identified by in-depth liquid chromatography-tandem mass spectrometry(LC-MS/MS) analysis.Our results provide a basis for further understanding the pathological significance of vinculin in the regulation of carcinogenesis,invasion and metastasis of colorectal tumors. In the present study, we aimed to global profile the proteins involved in colorectal carcinoma (CRC), in order to find clues to the pathological process of CRC. Pairs of malignant tissues and their adjacent healthy tissues from patients with colorectal cancer were subject to differential proteomics analysis. Two dimensional electrophoresis coupled with mass spectrometry (2-DE / MS) was used to identify differentially expressed proteins between pairs of tissue samples. A list of proteins relevant to the progression of colorectal tumors was identified by two dimensional gel electrophoresis -DE) -based proteomics approach. Among the identified proteins, vinculin was found to be remarkably down-regulated in colorectal carcinoma tissues. In addition, the three phosphorylation modifications within the isolated vinculin were identified by in-depth liquid chromatography-tandem mass spectrometry LC-MS / MS) analysis.Our results provide a basis for further understanding the pathological significance of vinculin i n the regulation of carcinogenesis, invasion and metastasis of colorectal tumors.