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目的通过考察野菊花对人胃癌细胞株SGC-7901、人宫颈癌细胞株HeLa生长的促进或抑制作用,评价其寒热属性。方法用MTT比色法考察野菊花对HeLa细胞和SGC-7901细胞增殖的影响;倒置显微镜观察野菊花对细胞形态特征的影响;台盼蓝染色法分析野菊花对HeLa细胞和SGC-7901细胞的细胞毒活性。结果野菊花质量浓度≤20μg/mL时对细胞生长有促进作用,使细胞密度增加、结构致密、生长旺盛,单个细胞略变大。台盼蓝染色表明野菊花对两种细胞无细胞毒活性。结论野菊花药性温热,且对SGC-7901细胞、HeLa细胞无细胞毒活性。
Objective To investigate the promotion or inhibition of the growth of human gastric cancer cell line SGC-7901 and human cervical cancer cell line HeLa by evaluating the chilling and heating properties of wild chrysanthemum. Methods MTT colorimetric assay HeLa cells and SGC-7901 cell proliferation; microscope inverted microscope observation of wild chrysanthemum cells morphological characteristics; trypan blue staining analysis of wild chrysanthemum on HeLa cells and SGC-7901 cells Cytotoxic activity. Results When the concentration of wild chrysanthemum was less than or equal to 20μg / mL, the cell growth was promoted, the cell density increased, the structure was dense, the growth was vigorous, and the single cell slightly enlarged. Trypan blue staining showed that wild chrysanthemum had no cytotoxic activity on both cells. Conclusion The wild chrysanthemum is warm and has no cytotoxic activity on SGC-7901 cells and HeLa cells.