目的克隆恙虫病立克次体Ｇｉｌｉａｍ株５６－ｋＤａ蛋白基因，作为优化ＰＣＲ反应时的标准模板和为Ｇｉｌｉａｍ株５６－ｋＤａ蛋白基因的表达作准备。方法利用ＰＣＲ反应和Ｔ载体分别扩增和克隆目的基因片段，并用序列分析方法鉴定克隆基因的正确性。结果克隆的基因为恙虫病立克次体Ｇｉｌｉａｍ株５６－ｋＤａ蛋白基因。结论利用ＰＣＲ反应和Ｔ载体可以从少量的标本中，高效获得高纯度的目的基因。 Objective To clone the 56-kDa protein of Rickettsia tsutsugamushi Giliam strain as a standard template for optimizing PCR reaction and to prepare for the expression of the 56-kDa protein gene of Giliam strain. Methods PCR and T vector were used to amplify and clone the target gene fragments respectively. The correctness of the cloned genes was identified by sequence analysis. Results The cloned gene was Rickettsia tsutsugamushi Giliam strain 56-kDa protein gene. Conclusion High purity of the target gene can be efficiently obtained from a few samples by PCR and T vector.