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目的:研究乳腺良、恶性病变组织中人乳头瘤病毒(HPVs)感染情况。方法:收集新鲜乳癌组织标本73例和乳腺良性病变组织标本84例,采用PCR方法对乳腺良、恶性病变组织中HPVs进行检测和分型。结果:73例乳癌组织中,HPV16阳性率为76.7%(56/73),高危型HPVs(HPV16和59)总阳性率76.7%(56/73);其中HPV16单独感染20例,HPV16、59混合感染6例,HPV16、6/11混和感染28例,3者混和感染2例。84例乳腺良性病变组织中,HPV16阳性率为48.8%(41/84),高危型HPVs总阳性率为51.2%(43/84);其中HPV16单独感染15例,HPV59单独感染2例,HPV6/11单独感染2例,HPV16、6/11混和感染24例,3者混和感染2例。乳腺良、恶性病变组织中均未检测到HPV18、31、33、52和58。乳癌组织中HPV16阳性率和高危型HPVs总阳性率高于乳腺良性病变(χ2分别为12.879,10.921,P<0.01)。浸润性导管癌组织中HPV16阳性率为86.0%(43/50),高于浸润性小叶癌组织中的56.6%(13/23)(χ2=7.663,P<0.01)。结论:HPV16在乳癌发生发展中可能具有重要作用,与乳癌组织学类型有关;其与其他HPVs亚型的混合感染值得重视。
Objective: To study the infection of human papillomavirus (HPV) in benign and malignant breast tissues. Methods: 73 cases of fresh breast cancer tissue samples and 84 cases of benign breast lesions were collected. HPVs were detected and classified by PCR in benign and malignant breast lesions. Results: The positive rates of HPV16 in 76 cases of breast cancer were 76.7% (56/73) and 76.7% (56/73) in high-risk HPVs (HPV16 and 59) Infected 6 cases, HPV16,6 / 11 mixed infection in 28 cases, 3 were mixed infection in 2 cases. Among 84 cases of benign breast lesions, the positive rate of HPV16 was 48.8% (41/84), while the high-risk HPVs were 51.2% (43/84) in total. Among them, 15 were infected with HPV16, 2 were infected with HPV59 alone, Infection alone in 2 cases, HPV16, 6/11 mixed infection in 24 cases, 3 were mixed infection in 2 cases. Breast of benign and malignant lesions were not detected in HPV18, 31,33,52 and 58. The positive rates of HPV16 and HPV in breast cancer were higher than those in benign breast lesions (χ2 = 12.879 and 10.921, respectively, P <0.01). The positive rate of HPV16 in invasive ductal carcinoma was 86.0% (43/50), which was higher than that in invasive lobular carcinoma (56.6%, 13/23) (χ2 = 7.663, P <0.01). Conclusion: HPV16 may play an important role in the development of breast cancer, which is related to the histological type of breast cancer. The mixed infection with other HPVs is worthy of attention.