Effect of Macronutrient on Shoot Growth and Catechins Production in Hadong Tea Plant in Vitro Cultur

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For efficient propagation,the in vitro propagation method was established.Among several culture media,shoot number and shoot length were high on MS and White medium,while the plantlets cultured on NN and B5 medium showed poor growth than others.The vigorous rooting ability occurred on culturing of explants for 4 weeks on White medium,with about 5.5 roots per plantlet.In order to investigate the effect of different macro nutrients,several macro nutrients(N,P,K,Mg and Ca) were added at levels of 1 /4,1 /2,1,2 and 4 strength into MS basal medium and determined shoot growth and catechins contents after 4 weeks of culture.Low concentration of nutrients resulted in a marked increase in plant growth,whereas high level of nutrients inhibited plant growth.The catechins production was also affected by macronutrient concentration.Low concentration of macronutrient increased in catechins production,whereas high level of macronutrient inhibited plant growth except magnesium sources. For efficient propagation, the in vitro propagation method was established. Among several culture media, shoot number and shoot length were high on MS and White medium, while the plantlets cultured on NN and B5 medium showed poor growth than others. on culturing of explants for 4 weeks on White medium, with about 5.5 roots per plantlet. In order to investigate the effect of different macro nutrients, several macro nutrients (N, P, K, Mg and Ca) were added at levels of 1 / 4,1 / 2, and 4 strength into MS basal medium and determined shoot growth and catechins contents after 4 weeks of culture. Low concentration of stem resulted in a marked increase in plant growth, whereas high level of plants inhibited plant growth L c concentration production of macronutrient increased in catechins production, high high of macronutrient inhibited plant growth except magnesium sources.
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