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目的探讨甘草对过氧化氢(H2O2)诱发人HepG2细胞DNA损伤的保护作用。方法培养的HepG2细胞经甘草(2,4,8和16 g/L)单独处理或甘草与H2O2(2 g/L+500μmol/L,10 g/L+500μmol/L)同时处理2 h后,分别运用彗星试验(comet assay)结合彗星图像分析软件(CASP)分析细胞尾部DNA百分率变化。结果甘草单独处理的HepG2细胞尾部DNA百分率没有明显变化,与不处理对照组相比,差异无统计学意义(P>0.05);但甘草与H2 O2同时处理的HepG2细胞尾部DNA百分率显著降低,与H2O2(500μmol/L)单独处理对照组相比,差异有统计学意义(P<0.01)。结论甘草本身不能诱发HepG2细胞DNA损伤,但甘草对H2 O2诱发HepG2细胞DNA损伤有保护作用。
Objective To investigate the protective effect of licorice root on DNA damage induced by hydrogen peroxide (H2O2) in human HepG2 cells. HepG2 cells were treated with licorice (2, 4, 8 and 16 g / L) alone or with licorice and H2O2 (2 g / L + 500μmol / L and 10 g / L + 500μmol / L) The comet assay (comet assay) combined with Comet Assay Software (CASP) was used to analyze the percentages of tail DNA. Results The percentage of tail DNA of HepG2 cells treated with licorice root alone did not change significantly (P> 0.05), but the percentage of tail DNA of HepG2 cells treated with licorice and H2 O2 was significantly lower than that of untreated control group H2O2 (500μmol / L) alone compared with the control group, the difference was statistically significant (P <0.01). Conclusion Licorice can not induce DNA damage in HepG2 cells by itself, but Glycyrrhiza uralensis has a protective effect on DNA damage induced by H2O2 in HepG2 cells.