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本文用苯肼诱发大白鼠溶血性贫血,测定其红细胞中的甘氨酸含量及与可使代谢有关酶系的活性。发现:苯肼可使大白鼠红细胞中甘氨酸水平明显升高,而血浆含量不变;用反复放血诱发大鼠网织红细胞增生时,其红细胞中甘氨酸含量也升高,但不如前者明显。苯肼可使红细胞中丙氨酸、谷氨酸或谷酰胺与乙醛酸的转氨基作用明显加强;而失血性贫血无此种改变。苯肼还可使红细胞中δ-ALA合成酶活性明显升高,此酶活力增强与甘氨酸合成有关的转氨酶的活力升高一样,均为血红素合成的必需条件。上述结果表明大鼠在严重贫血时体内的代偿能力很强。
In this paper, phenylhydrazine-induced hemolytic anemia in rats, determination of glycated content in erythrocytes and metabolism-related enzyme activity. It was found that Phenylhydrazine could significantly increase the level of glycine in erythrocytes of rats, while the plasma content remained unchanged. The glycine content of erythrocytes was also increased when reticulocyte was induced by repeated bloodletting, but not as obvious as the former. Phenylhydrazine red blood cells can make alanine, glutamic acid or glutamine and glyoxylate transamination significantly enhanced; and no such changes in hemorrhagic anemia. Phenyl hydrazine can also make the erythrocyte δ-ALA synthase activity was significantly increased, the enzyme activity and glycine synthesis of transaminases associated with increased vitality are essential for heme synthesis. The above results show that the rats have a very high degree of compensatory ability in severe anemia.