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目的 :调查重型病毒性肝炎经输血传播病毒 (transfusion transmitted virus,TTV)的感染状况。方法 :设计 TTV基因的特异性引物 ,采用巢式 PCR法对 37例重型肝炎进行血清中 TTV DNA的检测 ,并对 TTV DNA阳性病例进行临床资料分析。结果 :37例重型肝炎中 9例血清 TTV DNA阳性 (2 4.3% ) ,其中 7例重叠感染 HBV,1例重叠感染 HCV,1例为单纯 TTV感染 ;9例中急性重型肝炎 1例 ,亚急性重型肝炎 6例 ,慢性重型肝炎 2例 ;9例中 5例死亡 (5 5 .6 % ) ,1例有明确输血史。结论 :TTV可在各型重型肝炎外周血中检出 ,TTV可能是导致重型肝炎和促进肝功能衰竭的重要因素之一。
Objective: To investigate the infection status of transfusion transmitted virus (TTV) in severe viral hepatitis. Methods: The specific primers of TTV gene were designed. TTV DNA was detected by nested PCR in serum of 37 patients with severe hepatitis, and the clinical data of TTV DNA positive cases were analyzed. Results: Serum TTV DNA was positive in 9 out of 37 cases of severe hepatitis (2 4.3%), of which 7 cases were overlapped with HBV infection, 1 case was superinfected with HCV and 1 case was pure TTV infection. 9 cases were acute severe hepatitis with subacute 6 cases of severe hepatitis, 2 cases of chronic severe hepatitis; 5 cases of 9 cases died (55.6%), 1 case of a clear history of blood transfusion. Conclusion: TTV can be detected in the peripheral blood of various types of severe hepatitis. TTV may be one of the important factors leading to severe hepatitis and promoting liver failure.