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Ⅱ型胶原是人体软骨基质内的主要有机成分,其生化组成及数量的变化在一定程度上可反映出疾病的病因及发展过程,本实验以死婴软骨作材料,用限制性胃酶降解法提取可溶成分,用4M盐酸胍分离法、DEAE—纤维素除去蛋白多糖,盐析至3.2M以除去Ⅰ型胶原,用0.01M磷酸氢二钠除去降解部分,经SDS-PAG电泳,溴化氰降解及氨基酸分析证明所提Ⅱ型胶原符合文献要求,和国外同类产品相同。
Type II collagen is the main organic component in the cartilage matrix of human body. The changes of its biochemical composition and quantity can reflect the etiology and development of the disease to a certain extent. In this experiment, the dead baby cartilage was used as the material, The soluble fraction was extracted and the proteoglycan was removed with 4M guanidine hydrochloride, DEAE-cellulose, salted out to 3.2 M to remove type I collagen, and the degraded fraction was removed with 0.01 M dibasic sodium phosphate. SDS- Cyanide degradation and amino acid analysis showed that the type Ⅱ collagen in line with the literature requirements, and similar foreign products.