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采用HPLC法测定血清中司帕沙星的浓度.血清标本经甲醇沉淀蛋白,高速离心后取上清液直接进样.色谱条件以PE-pack C_(18)柱(5μm,4.6mm*150mm)为色谱柱,甲醇-0.2mol?L~(-1)乙酸铵溶液(pH2.7)(30:70)为流动相,检测波长为298nm血清中药物的最低检测浓度为0.04μg?ml~(-1).在0.05~2μg?ml~(-1)血药浓度范围内峰高与药物浓度线性关系良好,Y=5.835 1X-0.030 6,r=0.999 3.相对回收率为91.9％~103.4％,绝对回收率为91.5％~93.6％.日内RSD为1.5％~1.9％,日间RSD为1.9％~4.5％.方法简单、快速、准确,可用于司帕沙星的临床药动学研究.“,”HPLC method was established for the determination of serum concentration of sparfloxacin. The protein in serum sample was precipitated by methanol, and the supernatant was directly injected into HPLC after high speed centrifugation. Chro-matographic column was a PE-pack C18 cartridge column (5μm, 4.6mm*150mm). The mobile phase consisted of methanol-0.2mol·L-1 ammonium acetate solution (pH2.7)(30:70). The column eluant was determined at 298nm. The minimal drug concentration detected in serum was 40 ng·ml-1. A good linean relationship, existed between the response (Y) and sparfloxacin concentration in the range from 0.05 to 2.0 μg·ml-1. Y = 5.835X-0.030 6, r=0.999 3. The relative recoveries ranged from 91.6% at three different concentrations. The intraday RSD ranged from 1.5% to 1.9%, and interday RSD from 1.9% to 4.5%. this method is simple, rapid and accurate, it can be used for the investigation of clinical pharmacokinetics of sparfloxacin.