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目的:以响应面法优化黑果枸杞多糖提取工艺,正交法优化显色条件。方法:在单因素考察的基础上,选定提取温度、提取时间和料液比3个因素为自变量,以多糖提取率为响应值,利用响应面法建立数学模型,获得最佳工艺。选择对多糖含量测定结果产生显著影响的4个显色因素:5%苯酚用量、浓硫酸用量、反应温度、水浴显色时间进行正交试验。结果:最优工艺为:提取时间2h,提取温度90℃,液料比30∶1,在此优化条件下,平均提取率为3.21%,得率为5.0%。与理论值的相对误差为0.31%;最佳显色反应条件是质量分数为5%苯酚加入量为1.5mL,浓硫酸5mL,显色温度为90℃,水浴显色时间为30min。结论:响应面法优化黑果枸杞多糖提取工艺及正交法优化显色条件,方法简便,结果可靠。
Objective: To optimize the extraction process of Lycium barbarum polysaccharides by response surface methodology and optimize the conditions of color development by orthogonal test. Methods: On the basis of single factor study, three factors including extraction temperature, extraction time and solid-liquid ratio were selected as independent variables. The polysaccharide extraction rate was used as the response value, and the mathematical model was established by response surface methodology to obtain the best technology. Four color factors that significantly affect the determination of polysaccharide content were selected: 5% phenol, concentrated sulfuric acid, reaction temperature and water bath color development time. Results: The optimal extraction process was as follows: the extraction time was 2h, the extraction temperature was 90 ℃, the ratio of liquid to material was 30:1. Under the optimized conditions, the average extraction rate was 3.21% and the yield was 5.0%. The relative error with theoretical value was 0.31%. The best color reaction condition was that the mass fraction of 5% phenol was 1.5mL, the concentration of sulfuric acid was 5mL, the color temperature was 90 ℃, and the color developing time was 30min. Conclusion: Optimization of the extraction conditions of LBP and optimization of color conditions by orthogonal test is achieved by response surface methodology. The method is simple and the results are reliable.