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A conductometric nitrate biosensor based on methyl viologen/Nafion/nitrate reductase(NR) interdigitated electrode for heavy metal determination was proposed.The activity of NR(EC 1.6.6.2) from Asper gillusniger(A.niger) was assayed as a function of metal concentration in the presence of Cu2+,Zn2+,Cd2+ and Pb2+.Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition determination of heavy metal ions.Detection limits for Cu2+,Zn2+,Cd2+ and Pb2+ were about 0.05,0.5,0.1 and 1.0 μmol/L,respectively.The results show that NR activity could not be regained after exposure to Cu2+,but could be partially recovered after exposure to Zn2+,Pb2+ and Cd2+.
A conductometric nitrate biosensor based on methyl viologen / Nafion / nitrate reductase (NR) interdigitated electrode for heavy metal determination was proposed. Activity of NR (EC 1.6.6.2) from Aspergillus niger (A. niger) was assayed as a function of Metal concentration in the presence of Cu2 +, Zn2 +, Cd2 + and Pb2 + .Parameters influencing the performance of the biosensor were optimized for the application of it in the inhibition of heavy metal ions. Detection limits for Cu2 +, Zn2 +, Cd2 + and Pb2 + were about 0.05 , 0.5, 0.1 and 1.0 μmol / L, respectively. These results show that NR activity could not be regained after exposure to Cu2 +, but could partially recover after exposure to Zn2 +, Pb2 + and Cd2 +.