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人羊膜间充质干细胞(h AMSCs)具有自我增殖和多向分化潜能,有望为干细胞移植性治疗提供新来源,是病变组织器官损伤修复的理想种子细胞.但目前关于h AMSCs对肝损伤的修复机制仍不十分清楚.本研究采用胰蛋白酶-胶原酶消化法从羊膜组织中分离、纯化了间充质干细胞.免疫荧光检测表面标记波形丝蛋白(vimentin)和阶段特异表达抗原4(SSEA-4)均呈阳性.h AMSCs表达CD29、CD49d、CD73表面抗原,但不表达骨髓间充质表面抗原CD34、CD45和人类白细胞抗原DR位点(HLA-DR).实时定量PCR和Western印迹检测揭示,h AMSCs移植后可提高受损肝组织中肝细胞生长因子(HGF)和沉默信息调节因子1(SIRT-1)的表达,抑制α-平滑肌肌动蛋白(α-SMA)和周期性蛋白依赖性激酶抑制因子(P27kip1)的表达.因为上述蛋白质分子涉及肝细胞增殖、再生、凋亡调节,抑或肝纤维化过程,因此h AMSCs移植后所引起的上述分子表达变化可改善四氯化碳(CCL4)诱导的肝损伤,抑制肝细胞凋亡,促进肝细胞有丝分裂,对肝损伤有一定的修复作用.该研究为进一步探索调控肝再生、损伤修复信号通路(机制)及预防肝纤维化提供了新启示.
Human amniotic mesenchymal stem cells (hMSCs) have the potential of self-proliferation and multi-directional differentiation and are expected to provide a new source for stem cell transplantation and are the ideal seed cells for organ damage repair of diseased tissues. However, the current research on h AMSCs repair of liver injury Mechanism is still unclear.In this study, mesenchymal stem cells were isolated and purified from amniotic tissue by trypsin-collagenase digestion method.The surface markers of vimentin and phase-specific antigen 4 (SSEA-4 ) .h AMSCs express CD29, CD49d and CD73 surface antigen, but do not express bone marrow mesenchymal surface antigen CD34, CD45 and human leukocyte antigen DR site (HLA-DR) .Real-time quantitative PCR and Western blot analysis revealed that, h AMSCs transplantation can increase the expression of hepatocyte growth factor (HGF) and silencing information regulator 1 (SIRT-1), inhibit the α-smooth muscle actin (α-SMA) and the cyclic protein-dependent Expression of kinase inhibitor (P27kip1). Since these protein molecules are involved in hepatocyte proliferation, regeneration, apoptosis regulation, or hepatic fibrosis, the above-mentioned molecular expression caused by h AMSCs after transplantation Changes can improve liver injury induced by carbon tetrachloride (CCL4), inhibit hepatocyte apoptosis, promote mitosis of liver cells and repair liver damage.This study is to further explore the mechanism of regulating liver regeneration, injury and repair signal pathways ) And prevention of liver fibrosis provides a new revelation.