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本文报道,不同发育阶段的体外连续培养和取自感染夜猴的恶性疟原虫无性红内期经代谢标记后进行蛋白抗原比较分析结果。实验用的恶性疟原虫Uganda-Palo-Alto株已在体外连续培养8年以上。俟原虫血症达3～5%时,在含20%人血清的RPMI-1640培养基内加4mM秋水仙硷培养24小时,每5小时用不含秋水仙硷的培养基更换一次,48小时可完成体外培养原虫的同步化。每一种主要含环状体、滋养体及裂殖体的同步化培养基用~3H-异白氨酸按1.0mCi:0.5ml压积红细胞的比率进行代谢标记。另以同株恶性疟原虫感染夜猴,俟原虫血症达25～30%时采血,以RPMI-1640洗3次,过纤维素
This paper reports, in different stages of continuous culture in vitro and from infected with the late-stage monkey ape malaria parasite asexual metabolic markers by protein antigen comparison analysis results. The experimental Uganda-Palo-Alto strain of Plasmodium falciparum has been continuously cultured in vitro for more than 8 years. When the parasitemia is up to 3 to 5%, the cells are cultured in RPMI-1640 medium containing 20% human serum for 4 hours with 4mM colchicine and once every 5 hours for 48 hours with colchicine-free medium Can be completed in vitro protozoan synchronization. Synchronization media, each containing mainly annulus, trophozoites and schizonts, was metabolically labeled with ~ 3H-isoleucine at a rate of 1.0 mCi: 0.5 ml hematocrit. The other with the same strain of Plasmodium falciparum infection in late-night monkeys, protozoal parasite blood 25 to 30% when the blood, washed three times with RPMI-1640, cellulose